The Yellow River Basin (Jiyang Section) is rich in geothermal resources. It is of great significance to elucidate the genetic mechanism of geothermal fields and the exploitation and utilization of regional geothermal water resources for high-quality development of the Yellow River basin. Taking thermal reservoir of limestone in the south of Qiguang fault in the Yellow River basin (Jiyang section) as the research object, hydrochemical analysis and isotope analysis have been carried out. It is showed that hydrochemical types of geothermal water in the study area are mainly SO4·Cl-Ca·Na type, SO4-Ca·Na type, HCO3·SO4-Ca·Mg type and SO4-Ca type. The geothermal water is generally replenished by the infiltration of atmospheric precipitation, and the elevation range of the geothermal water recharge area is 497.81~2117.97m. The geothermal water belongs to the mixed water of submodern and recent replenishment and modern water.