On the basis of biostratigraphic information of strata profile with the scale of 1∶50000 and 1∶200000, combining with field trace, and using proximate isochronism of biostratigraphic zone, second order sequence boundary in middle part of Cambrian in Luxi area is studied emphatically. It is regarded that this boundary occurs in lower part of shale in Mantou formation, upper part of shale in Honghe formation and Mantou formation and lower part of limestone in Zhangxia formation. It occurs in Luxi area widely with stable strend. It transits from deceptive conformity to paraconformity frome NW to SE, and has an evident sedimentary break in west part. Sequence boundary belongs to Ⅱ type discontinuity.